Journal: Biology of reproduction

Article Title: Distribution of immune cells in the epididymis of the aging Brown Norway rat is segment-specific and related to the luminal content.

doi: 10.1095/biolreprod61.3.705

Figure Lengend Snippet: FIG. 1. Light micrographs showing sec- tions of the epididymis of the Brown Nor- way rat stained with an antibody for monocytes-macrophages (ED1). A–C) Cor- pus, D–F) distal cauda. A, D) 3 mo, B, E) 18 mo with numerous spermatozoa in the lumen, C, F) 18 mo with occasional sper- matozoa in the lumen. lu, Lumen; it, inter- tubular space; p, principal cells; b, basal cells; c, clear cells; bm, basement mem- brane. Arrows, ED1-positive cells; aster- isks, halo cells. Scale bar, A–F 5 64 mm.

Article Snippet: Mouse anti-rat ED1 (Serotec USA, Washington, DC) was used at a dilution 1/100; this antibody recognizes a cytoplasmic antigen in monocytes and most macrophages.

Techniques: Staining

Journal: Biology of reproduction

Article Title: Distribution of immune cells in the epididymis of the aging Brown Norway rat is segment-specific and related to the luminal content.

doi: 10.1095/biolreprod61.3.705

Figure Lengend Snippet: FIG. 5. Light micrographs showing sec- tions of the corpus of the epididymis of Brown Norway rats. A, C) Three mo; B, D) 18 mo. Section stained with an antibody for A and B GST Yf and C and D ED1. Clear arrows, basal cells; dark arrows, ED1-positive cells; other labels as in Fig- ure 1. Scale bar, A–D 5 64 mm.

Article Snippet: Mouse anti-rat ED1 (Serotec USA, Washington, DC) was used at a dilution 1/100; this antibody recognizes a cytoplasmic antigen in monocytes and most macrophages.

Techniques: Staining

Journal: Molecular Vision

Article Title: P2X 7 receptor activation may be involved in neuronal loss in the retinal ganglion cell layer after acute elevation of intraocular pressure in rats

doi:

Figure Lengend Snippet: Representative photomicrographs of double immunostaining of the P2X 7 receptor and CD68 in the normal retina and in the retina on days 1, 2, 3, and 7 after intraocular pressure elevation. P2X 7 -positive cells were also stained with anti-CD68 in the ganglion cell layer (GCL). Bar=100 µm.

Article Snippet: For this, mouse Alexa Fluor 488–labeled anti-TUJ1 monoclonal antibody (A488–435L, Covance Research Products, Princeton, NJ) and mouse anti-CD68 (MAB1435, Merck Millipore, Billerica, MA) were used.

Techniques: Double Immunostaining, Staining

Journal: Molecular Vision

Article Title: P2X 7 receptor activation may be involved in neuronal loss in the retinal ganglion cell layer after acute elevation of intraocular pressure in rats

doi:

Figure Lengend Snippet: Representative photomicrographs of double immunostaining of CD68 and TNF-α or interleukin-1β (IL-1 β) in the normal retina and the retina on day 2, with and without treatment of oxidized adenosine triphosphate (OxATP) at 30 µM just after intraocular pressure (IOP) elevation. A: TNF- α, B: IL-1 β. Immunoreactivities of tumor necrosis factor-α (TNF-α) and IL-1β were upregulated in the ganglion cell layer (GCL) and inner plexiform layer (IPL) cells on day 2 after IOP elevation; they were subsequently suppressed by treatment with OxATP. Bar=100 µm.

Article Snippet: For this, mouse Alexa Fluor 488–labeled anti-TUJ1 monoclonal antibody (A488–435L, Covance Research Products, Princeton, NJ) and mouse anti-CD68 (MAB1435, Merck Millipore, Billerica, MA) were used.

Techniques: Double Immunostaining